Observations on protective immunity in mice induced by recombinant Schistosoma japonicum Sj14FABP through several adjuvant formulations
- VernacularTitle:重组日本血吸虫脂肪酸结合蛋白在不同佐剂条件下的免疫效果观察
- Author:
Jinming LIU
;
Zhiqiang FU
;
Youmin CAI
;
Yaojun SHI
;
Hao LI
;
Ke LU
;
Jiaojiao LIN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Sj14;
Adjuvant;
Bacillus Calmette-Guerin(BCG);
Immunostimulating complex (ISCOM)
- From:
Chinese Journal of Schistosomiasis Control
2007;19(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the protective immunity in mice induced by recombinant Schistosoma japonicum Sj14FABP through several adjuvant formulations. Methods The recombinant Schistosoma japonicum Sj14FABP was prepared by expression in E. coli as a GST fusion protein (rSj14/GST) and used to vaccinate outbred Kunming mice by using complete Freund's adjuvant (FCA)/incomplete Freund's adjuvant (FIA), Bacillus Calmette-Guerin (BCG) and the immunostimulating complex (ISCOM) as adjuvant respectively. Results The purified recombinant protein rSj14/GST was immunogenic in mice, and 34.3% and 36.0% worm reduction rates were obtained in outbred Kunming mice immunized intradermally with BCG adjuvant and immunized subcutaneously with ISCOM adjuvant respectively, compared with non-vaccinated control group. However, intramuscularly vaccination with rSj14/GST in FCA/FIA was not protective, although the high level of IgG antibody was induced. Conclusion Both BCG and ISCOM are suitable adjuvants for rSj14/GST.
