Citrus fiavonoids simultaneous HPLC analysis
- VernacularTitle:柑橘类黄酮成分的同步HPLC检测
- Author:
Chongwei ZHANG
;
Dongzhi WEI
;
Wenyu ZHOU
;
Yanhua LU
- Publication Type:Journal Article
- Keywords:
Citrus aurantium L.;
flavanone glycoside;
polymethoxylated flavone;
naringin;
hesperidin;
neohesperidin;
sinensetin;
nobiletin
- From:
Chinese Traditional Patent Medicine
2007;29(1):99-104
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids, naringin, hesperidin, neohesperidin, sinensetin and nobiletin. METHODS:Gradient elution with non-salt mobile phase ( methanol and water only) HPLC method on a Kromasil column ( 100-5C18-250A, 4.6 mm ×250 mm, 5 μm, C18 reverse phase) with peaks identification through DAD full UV wavelength scan. UV 284 nm and 332 nm profiles were observed. RESULTS: Satisfactory resolution, linearity, 95%～ 102% of recovery and 1.88 ～ 2.93% of repeatability were obtained for those five citrus flavonoids. Content of 6 Citrus aurantium L. based TCM ingredients were analyzed and identified. CONCLUSION: Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.
