Effect of berberine on invasion and migration of PG cells from a high metastatic human giant lung carcinoma cell line
- VernacularTitle:小檗碱抑制高转移性人巨细胞肺癌PG细胞侵袭和迁移的研究
- Author:
Yu HAO
;
Bowen XU
;
Hong ZHENG
;
Qian WANG
;
Quanying QIU
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Berberine;
Neoplasm invasiveness;
Neoplasm metastasis;
Gelatinase A;
Tissue inhibitor of metalloproteinase - α
- From:
Chinese Journal of Pathophysiology
2007;23(3):474-478
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of berberine(Ber) on invasion and migration of PG cells from a high metastatic human giant lung carcinoma cell line and to explore its mechanism. METHODS: Agarose drop method was used to detect PG migration; transwell cabin with FN in lower chamber was adopted to detect PG chemotaxis. PG adhesion to FN and martrigel was detected by MTT; PG invasive ability was determined by transwell cabin covered with martrigel. Expression of MMP2/TIMP2 protein and mRNA were detected by quantitative immunocytochemical method and RT - PCR respectively. RESULTS: After PG was treated by Ber( 10 mg/L) for 24 h: 1 ) migration distance of Ber- treated PG cells was markedly shorter than that of control cells (P ＜0. 01 ) and the number of passed membrane cells towards FN was much fewer than that of control cells ( P ＜ 0. 01 ); 2) PG adhesion to FN and martrigel was inhibited remarkably by Ber compared with control PG; 3) the migration of PG cells through the martrigel -coated transwell was significantly inhibited by the addition of Ber; 4) MMP2 expression was reduced significantly(P ＜0. 01 ), while the TIMP2 expression showed up - regulating tendency, but had no differences compared with control group (P ＞ 0. 05). The MMP2/TIMP2 ratio was decreased; 5 )the MMP2 mRNA/TIMP2 mRNA ratio was decreased by Ber. CONCLUSION: Inhibition of cell migration, adhesion to ECM and invasion into ECM of tumor cells and regulation of homeostasis between MMPs and TIMPs to maintain ECM integrity may be the basic mechanism of inhibitive effect of Ber on invasion and metastasis of tumors.
