Acrolein-induced cell apoptosis in adult mice cardiomyocytes
- VernacularTitle:丙烯醛致成年小鼠心肌细胞凋亡的作用
- Author:
Lijuan WANG
;
Jian HU
;
Guoxian QI
- Publication Type:Journal Article
- Keywords:
acrolein;
oxygen free radical;
calcium;
adult mice cardiomyocytes;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2007;28(3):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the responsiveness of intracellular oxygen free radical and calcium on acrolein exposure and acrolein-induced cardiomyocytes apoptosis. Methods The viable adult mice cardiac myocytes were isolated by modified langendorff methods. We have examined the intracellular oxygen free radical and calcium concentration using DCF and Fura-2 AM, and the cardiomyocytes viability with WST assay. Are evaluated the DNA ladder pattern and cell apoptotic morphology on the adult mice cardiomyocytes that are exposed to acrolein. Results Our results show that acrolein can increase markedly the intracellular oxygen free radical and calcium concentration, that reach 12 fold and twofold respectively compared to the resting value when the cells were exposed to 1 μmol/L of acrolein. Moreover, the injury induced by acrolein in cardiac myocytes is concentration-dependent. The cardiomyocytes viability treated with 25, 50, 100 μmol/L of acrolein respectively were significantly lower compared to controls (P ＜ 0.01 ). DNA ladder pattern and apoptotic morphological changes were found after being exposed to acrolein in the adult mice cardiomyocytes. Conclusion It is concluded that acrolein induces adult mice cardiomyocytes apoptosis, and it may be due to the increased intracellular oxygen free radicals and calcium concentration.
