Recombinant Human PLD2(rhPLD2)May Significantly Inhibit Expression of GPI-PLD of Guinea Pigs of Chronic Asthma in vivo
- VernacularTitle:重组人磷脂酶D2在体内能明显抑制慢性哮喘豚鼠血清中GPI-PLD的表达
- Author:
Ling ZHU
;
Chuanxing YU
;
Weibin ZOU
;
Xiaoli HE
;
Junjin LIN
- Publication Type:Journal Article
- Keywords:
rhPLD2;
GPI-PLD;
chronic asthma;
guinea pig
- From:
Chinese Journal of Biochemistry and Molecular Biology
2007;23(2):116-121
- CountryChina
- Language:Chinese
-
Abstract:
The effect of recombinant human phospholipase D2(rhPLD2)in vivo was investigated on the secretion of serum glycosyl phosphatidylinositol-specific phospholipase D(GPI-PLD)in guinea pigs of chronic asthma．Ater treating the guinea pigs attacked by chronic asthma with rhPLD2,the GPI-PLD activity detection was canrried out by phase separation of human placental alkaline phosphatase in Triton X-114．Compared with the healthy guinea pigs(NS group),the serum GPI-PLD in the guinea pigs of chronic asthma are much higher than that of control groups,P≤0．01．Our results showed that rhPLD2 could significantly reduce the secretion of GPI-PLD when the guinea pigs were attacked by chronic asthma．
