Structural aberration of human sperm chromosomes and the effects of phenytoin sodium
- VernacularTitle:人精子染色体结构畸变与苯妥英钠的影响
- Author:
Huaqing MENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;11(4):798-800
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: If the generative cell DNA of each grade is damaged or mutated, it is possible to transmit to the further generations by means of fertilized ova. As a traditional antiepileptic, the mutagenic effects of phenytoin sodium on so matic cells had been confirmed by many researches, but it is still unknown whether phenytoin sodium has the mutagenic effects on generative cells. OBJECTIVE: To detect the mutagenic effects of phenytoin sodium on human sperm chromosomes. DESIGN: In present study we measured human sperm chromosomes in vitro by means of randomized control observa tion. SETTING: Mental Health Center of the First Affiliated Hospital, Chongqing University of Medical Sciences. MATERIALS: Phenytoin sodium was purchased from Sigma Company. The human sperms were collected from the healthy males who had not contacted with any physicochemical mutagen within recent 6 months. Ova were collected from female golden hamsters of 6-8 weeks, which were purchased from Shanghai Institute of Family Planning Science. Medium for washing sperms and ova was the BWW solution containing 0.3% human serum albumin (HSA); Medium for capacitation was the BWW solution containing 3.5% HSA; Medium for post-fertilization was an oval one containing 10% hamster serum. METHODS: After washing, centrifugation and capacitation, the sperms were made into suspension and dispended into 5 centrifuge tubes (5 mL each): Bleomycin A5 (40 mg/L) was added in the first tube as positive control group, phenytoin sodium (10, 20 and 40 mg/L) were added in three tubes respectively, another tube did not contain any reagent as blank control. Hamster ova without pellucid zone were prepared, and equally divided into five portions, which were mixed with the above-mentioned sperms in the five groups respectively, so as to make the hamster ova fertilize, finally human sperm chromosomes were prepared with the fertilized hamster ova. The rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages were examined. We examined the rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages. The effects of phenytoin sodium of three different concentrations on human sperm chromosomes were detected in vitro, and the results were compared with those in the positive control group and blank control groups. MAIN OUTCOME MEASURES: The structural aberration of sperm chromosomes, rate of aberrant sperm and number of chromosomal breakages were observed. RESULTS: ① Chromosomal structural aberration: The structural aberrations of sperm chromosomes including the chro mosomal breakage, monome breakage, fragments, crossing-over aberration, double centromere and ring-like chromo somes were observed in the phenytoin sodium groups, positive control group and blank control group, especially in the phenytoin sodium 40 mg/L group and positive control group. ② Rate of aberrant sperms and number of chromosomal breakages: The rate of aberrant sperms and number of chromosomal breakages were higher in the phenytoin sodium groups and positive control group than in the blank control group, but there was the significant differences between phenytoin sodium 40 mg/L group and positive control group (P ＜ 0.005, P ＜ 0.05-0.01). CONCLUSION: Phenytoin sodium has obvious influence on the structural aberration of human sperm chromosomes and may have mutagenic potential to human sperm cells.
