Determination of evodiamine by high performance liquid chromatography-tandem mass spectrometry and pharmacokinetic studies in rats
- VernacularTitle:LC-MS/MS法测定大鼠血浆中吴茱萸碱的浓度及其药代动力学研究
- Author:
Jihua XU
;
Wenying LIU
;
Feng ZHENG
;
Di SUN
;
Qian YANG
;
Jinhua RAO
- Publication Type:Journal Article
- Keywords:
evodiamine;
blood drug level plasma;
LC-MS/MS;
pharmacokinetic studies
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2007;12(4):427-433
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish an LC-MS/MS method for determination of evodiamine concentration in rat plasma and to study its pharmacokinetic profile in rats. METHODS: Six rats were administrated (i.g.) evodiamine at the dose of 100 mg/kg. Blood samples were collected from eye socket. Evodiamine concentration in rat plasma was determined by LC-MS/MS method. The pharmacokinetic parameters were calculated using DAS program. RESULTS: A good linear relationship was obtained in the concentration range (0.2-50.0 ng/mL) studied (r2=0.9997). Average recoveries ranged from 96.12% to 99.46%. Intra-and inter-day relative standard deviations were 4.61%-13.51% and 5.65%-11.49%, respectively. The main pharmacokinetic parameters of evodiamine were as follows: Cmax (5.3±1.5) ng/mL; tmax (22±8) min; t1/2 (451±176) min. CONCLUSION: A selective and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the quantification of evodiamine in rat plasma is developed and validated. This method is successfully applied for the pharmacokinetic studies of evodiamine in rats.
