Content of pseudoephedrine in blank plasma with high performance liquid chromatograpy
- VernacularTitle:高效液相色谱法测定空白血浆中伪麻黄碱的含量
- Author:
Gang LIU
;
Hui WANG
;
Benhong ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;11(8):1598-1600
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: There are several ways to measure content of pseudoephedrine, such as titration, spectrophotography,gas chromatography, capillary electrophoresis, high performance liquid chromatograpy (HPLC), combination of liquid chromatography, etc. Therefore, an exact method to measure concentration of medicine in blood is of significance for reasonable and safe administration in clinic.OBJECTIVE: To establish a reversed-phase-HPLC (RP-HPLC) method for determining the content of pseudoephedrine in blank plasma.DESIGN: Simple exponent study.SETTING: Department of Pharmacology, Renmin Hospital of Wuhan University.MATERIALS: The experiment was carried out in the Department of Pharmacology, Renmin Hospital of Wuhan University from May to July 2005. Pseudoephedrine was provided by Hubei Institute for Drug Testing. Blank plasma was provided by Central Blood Station of Xiaogan.METHODS: Standard plasma exponent was dispensed containing 50, 100, 200, 500, 750 and 1 000 μg/L pseudoephedrine, respectively. Pseudoephedrine was extracted from standard plasma exponent with organic solvent and its content was measured with HPLC. ZorbaxSB-C18 column (4.6 mm × 250 mm, 5 μm) was also used in this study [mobile phase: 0.2% phosphoric acid solution-acetonitrile (95:5); flow rate: 1.0 mL/min], and the detective wavelength was 194 nm.MAIN OUTCOME MEASURES: Veracity and feasibility through evaluating standard curve, precision and retrieved rate.RESULTS: Linearity ranged from 50 to 1 000 μg/L (r=0.999 3) and average retrieved rate was 79.20%. The relative standard deviation of within-day and between-day was less than 5%. The minimal detectable concentration of pseudoephedrine was 25 μg/L in plasma.CONCLUSION: RP-HPLC which is characterized by convenience and precision is suitable for researching pharmacokinetics and bioavailability of pseudoephedrine.
