Immunophenotypic markers in adult acute lymphoblastic leukemia: the prognostic significance of CD20 and TdT expression.
- Author:
Dae Young KIM
1
;
Han Seung PARK
;
Eun Ji CHOI
;
Jung Hee LEE
;
Je Hwan LEE
;
Mijin JEON
;
Young Ah KANG
;
Young Shin LEE
;
Miee SEOL
;
Young Uk CHO
;
Seongsoo JANG
;
Hyun Sook CHI
;
Kyoo Hyung LEE
;
Chan Jeoung PARK
Author Information
- Publication Type:Original Article
- Keywords: Acute lymphoblastic leukemia; Immunophenotypic markers; CD20; TdT
- MeSH: Adult*; Cytotoxins; Diagnosis; DNA Nucleotidylexotransferase; Flow Cytometry; Humans; Multivariate Analysis; Precursor Cell Lymphoblastic Leukemia-Lymphoma*; Retrospective Studies; Transplants
- From:Blood Research 2015;50(4):227-234
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Efforts to overcome poor outcomes in patients with adult acute lymphoblastic leukemia (ALL) have focused on combining new therapeutic agents targeting immunophenotypic markers (IPMs) with classical cytotoxic agents; therefore, it is important to evaluate the clinical significance of IPMs. METHODS: Baseline characteristics and clinical outcomes of patients with adult ALL were retrospectively analyzed. The percentage of blasts expressing IPMs at diagnosis was measured by multicolor flow cytometry analysis. Samples in which > or =20% of blasts expressed an IPM were considered positive. RESULTS: Among the total patient population (N=230), almost all (92%) were in first or second hematological complete remission (HCR) and 54% received allogeneic hematopoietic cell transplant (allo-HCT). Five-year hematologic relapse-free survival (HRFS) and overall survival (OS) rates were 36% and 39%, respectively, and 45.6% and 80.5% of patients were positive for the IPMs CD20 and terminal deoxynucleotidyl transferase (TdT), respectively. Expression of CD20, CD13, CD34, and TdT was associated with HRFS rate, and expression of CD20 and CD13 was associated with OS rate, as was the performance of allo-HCT. In multivariate analysis, positivity for CD20 (HRFS: hazard ratio [HR], 2.21, P<0.001; OS: HR, 1.63, P=0.015) and negativity for TdT (HRFS: HR, 2.30, P=0.001) were both significantly associated with outcomes. When patients were categorized into three subgroups according to positivity for CD20 and TdT, there were significant differences in HRFS and OS among the subgroups. CONCLUSION: Positivity for CD20 and TdT expression and clinical risk group were prognostic factors in adult ALL.
