p53 Anti-tumor Research in Bel-7402 by Using Human-derived Vector
- VernacularTitle:人源基因载体pHrn介导P53基因在Bel-7402中的抗癌研究
- Author:
Zhigang XUE
;
Jian LI
;
Biao YIN
;
Yakun ZHANG
;
Xionghao LIU
;
Qian PAN
;
Zhigao LONG
;
Heping DAI
;
Kun XIA
;
Lingqian WU
;
Desheng LIANG
;
Jiahui XIA
- Publication Type:Journal Article
- Keywords:
human-derived vector;
human telomeras reverse transcriptase (hTERT);
p53
- From:
Progress in Biochemistry and Biophysics
2007;34(5):465-470
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the tumor suppression effect of p53 with CMV enhancer and hTERT promoter mediated by human-derived vector pHrn in liver cancer cell Bel-7402, report plasmid pchEGFP, tumor suppressor plasmids pchp53Arg and pchp53Pro were constructed by inserting expression cassette CMVe+hTERTp+EGFP, CMVe+hTERTp+p53Arg and CMVe+hTERTp+p53Pro into pHrn respectively. 24 h after cell transfection by lipofectamine 2000, GFP expression pattern was analyzed through fluorescence microscope and flow cytometry; RT-PCR and Western blot were taken to study the p53 expression pattern. The cell apoptosis by Hoechst 33258 and Annexin V-FITC/PI staining was also studied. Results show that the expression of GFP and p53 protein in Bel-7402were detected, but apparent cell apoptosis could not be found. The recombinant p53 mediated by human-derived vector could express in Bel-7402, but no significant tumor suppression effect was detected, which might result from the down regulation effect of the wild type p53 on hTERT promoter.
