Factors Influencing Agrobacterium-mediated Transformation of Foxtail Millet (Setaria italica)
- VernacularTitle:根癌农杆菌介导转化谷子的影响因素
- Author:
Yinghui LIU
;
Jingjuan YU
;
Guangming AO
;
Qian ZHAO
- Publication Type:Journal Article
- Keywords:
foxtail millet;
transformation frequency;
Agrobacterium-mediated transformation;
influence factors
- From:
Chinese Journal of Biochemistry and Molecular Biology
2007;23(7):531-536
- CountryChina
- Language:Chinese
-
Abstract:
An efficient and repeatable approach in transforming the foxtail millet (Setaria italica ) using Agrobacterium LBA4404 horboring the plasmid pBI121 was established. Factors affecting transformation efficiency were investigated including the genotype, explants, the density of bacteria, the duration for inoculation and co-cultivation, and the concentration of acetosyringone in the medium. The maximum transformation conditions were: the callus induced from inflorescence was used as explant; the duration for inoculation with Agrobacterium at low cell density was 30-40 min; for the co-cultivation , the suitable concentration of acetosyringone in the medium was 0.1 mmol/L, and the duration was 2 days.
