EFFECT OF wt-P53 PROTEIN ON TELOMERASE ACTIVITY IN KELOID FIBROBLASTS
- VernacularTitle:wt-P53蛋白对人瘢痕疙瘩成纤维细胞端粒酶活性的影响
- Author:
Zhibo XIAO
;
Lijun HAO
;
Lihong REN
;
Wen TENG
- Publication Type:Journal Article
- Keywords:
wt-P53 protein;
Telomerase activity;
Keloid;
Fibroblasts
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2007;21(7):702-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts(KFBs). Methods The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis. KFBs were divided into 2 groups, the transfection group and the untransfection group. wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group. The KFBs untransfected with wt-p53 gene served as control (untransfection group). After 48 hours of transfection, the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method, respectively. The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.Results All the KFBs from 2 groups expressed wt-P53 protein. But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group. At the same time of high expression of wt-P53 protein, the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group( P＜0.05). Conclusion High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.
