Effect of Panax notoginseng on gastric mucosal injury, superoxide dismutase activity, malondialdehyde and nitric oxide contents in rats with water immersion restraint stress
- VernacularTitle:田七对水浸应激大鼠胃黏膜损伤及超氧化物歧化酶活性和丙二醛与一氧化氮含量的影响
- Author:
Hang YU
;
Qingbo JIA
;
Changqing XU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;11(25):5047-5049
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Panax notoginseng is an effective medicine for curing gastric mucosal ulcer. There are many clinical reports that Panax notoginseng protects gastric mucosa.OBJECTIVE: To investigate the protective mechanism of Panax notoginseng on the gastric mucosal injury induced by water immersion restraint stress in rats.DESIGN: A randomized controlled animal trial.SETTING: School of Basic Medical Sciences, Daqing Branch of Harbin Medical University.MATERIALS: The study was conducted in the laboratory of Department of Pathophysiology of Harbin Medical University from September 2004 to October 2005. Forty-eight Wistar rats were used, either male or female, weighing 180-230 g.METHODS: The 48 Wistar rats were randomly divided into six groups with 8 rats in each group: normal control group, stress model group, cimetidine treatment group, Panax notoginseng of low, middle and high-dose groups (4, 8, 12 mg/time). In the cimetidine treatment group, cimetidine tablets were grinded into powders, then mixed with distilled water to prepare into suspension (1 tablet:10 mL), which was perfused intragastrically (5 mL), 3 times a day; In the Panax notoginseng groups, the powders in Panax notoginseng capsules were mixed with distilled water to prepare into suspension of corresponding concentrations (0.8, 1.6, 2.4 g/L), then administrated the same as those in the cimetidine treatment group. Stress models in rats were established by means of water immersion restraint stress. The gross and pathohistological changes of gastric mucosa were observed, and the activity of superoxide oxidase (SOD) and contents of malondialdehyde (MDA) and nitric oxide (NO) were determined.MAIN OUTCOME MEASURES:①Gross and pathohistological changes of gastric mucosa;②Changes of MDA and NO contents and SOD activity in the homogenate of gastric mucosa.RESULTS: All the 48 rats were involved in the analysis of results. The gastric mucosal hemorrhage and erosion in the cimetidine treatment group were reduced obviously as compared with those in the stress model group, SOD activity was obviously decreased [(12.61±0.87), (1.03±0.60) mkat/g], whereas the NO content was a little higher [(5.76±1.35), (0.97±0.58) nmol/g]. The MDA content was obviously higher in the stress model group than in the normal control group [(3.10±1.13), (0.09±0.02) μmol/g, P＜0.01]. There were no obvious differences between the Panax notoginseng groups and the cimetidine treatment group except that the NO contents were decreased in the in Panax notoginseng groups.CONCLUSION: Gastric mucosal injury induced by water immersion restraint stress can be significantly protected by Panax notoginseng, which is not dose-dependent. The protective mechanism may be associated with that Panax notoginseng can eliminate the product of oxygen-derived free radicals, and it is not totally the same as that of cimetidine
