Plicamycin inhibits low potassium-induced apoptosis in cerebellar granule neurons of rat
- VernacularTitle:普卡霉素对大鼠小脑颗粒神经元低钾性凋亡的抑制作用
- Author:
Linguang SUN
;
Yijun HUANG
;
Xingwen SU
;
Tianhan KONG
;
Pengxin QIU
;
Guangmei YAN
- Publication Type:Journal Article
- Keywords:
cerebellum;
neurons;
potassium;
apoptosis;
plicamycin
- From:
Chinese Journal of Pharmacology and Toxicology
2006;20(5):361-366
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protection of plicamycin on apoptosis in cerebellar granule neurons (CGN) of rat. METHODS TUNEL, Hoechst 33258 staining, agarose gel electrophoresis and fluorescein diacetate staining were used to detect morphological and biochemical characteristics of apoptosis in primary rat CGN. RESULTS Being pre-incubated with plicamycin for 1 h and lasting for 24 h, rat CGN apoptosis induced by low potassium basal modified Eagle′s medium for 24 h was inhibited in a plicamycin concentration-dependent manner. This effective concentrations of plicamycin were from 50 to 200 nmol·L-1, and the maximum inhibitory rate of plicamycin on CGN apoptosis was near 80% at 200 nmol·L-1. CONCLUSIONPlicamycin inhibits rat CGN apoptosis induced by low potassium.
