Effect of human chemokine-like factor-1 expression in vivo on the development of systemic lupus erythematosus in BXSB mice
- VernacularTitle:人趋化因子样因子-1的体内表达对BXSB狼疮鼠发病影响的研究
- Author:
Zhiwei XU
;
Hongye DENG
;
Dalong MA
- Publication Type:Journal Article
- From:
Chinese Journal of Rheumatology
2001;5(3):162-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe into roles of hCKLF-1 in the development of systemic lupus erythematosus in BXSB mice in vivo.Methods The recombinant eukaryotic expression plasmid for hCKLF-1 (pCDI-hCKLF-1) was transferred and expressed in lupus-prone BXSB mice with the technique of muscle-mediated transgene by electric pulse;the levels of serum IgM and IgG anti-DNA antibodies as well as serum BUN and urine protein were detected.Results hCKLF-1 expression in vivo did not make any effects on the levels of IgM and IgG anti-DNA antibodies in female and male BXSB mice,but markedly enabled abnormal elevation of urine protein in male BXSB mice in short time,suggesting its ability for accelerating glomerulonephritis.Conclusion hCKLF-1 may be one of inflammation mediators,playing an important role in the lupus glomerulonephritis of male BXSB mice,and its target cells may be monocyte and macrophage.
