Determination of paeonol in human plasma by HPLC and its pharmacokinetic studies
- VernacularTitle:HPLC法测人血浆中丹皮酚浓度及其药代动力学研究
- Author:
Jing WU
;
Benjie WANG
;
Chunmin WEI
;
Xianglin KONG
;
Ruichen GUO
- Publication Type:Journal Article
- Keywords:
paeonol;
HPLC;
drug blood concentration;
phamacokinetics
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2007;12(8):935-938
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a sensitive HPLC method for determining the concentrations of paeonol in human plasma and to evaluate its pharmacokinetic characteristics. METHODS: A single oral dose of 160 mg paeonol capsules was given to 24 Chinese healthy volunteers. Paeonol was separated on a XB-C18 column with tetrahydrofuran-methanol-water-phosphonic acid (6∶60∶34∶0.1, V∶V) as mobile phase. The plasma concentrations of paeonol were determined and its pharmacokinetic parameters were calculated and evaluated using DAS 2.0. RESULTS: The linear range of the paeonol was 10-500 ng/mL and the determination limit was 10 ng/mL. The main pharmacokinetic parameters, as Cmax, tmax, t1/2,AUC0-3, AUC0-∞ after a single dose of paeonol capsules were (116±46)ng/mL,(1.02±0.13) h,(1.03±0.35) h, (174±45) ng/mL, (217±56) ng/mL,respectively. CONCLUSION: The HPLC method for determining paeonol concentration in plasma is rapid, sensitive and suitable for pharmacokinetic studies.
