Detection and significance of urokinase-type plasminogen activator and its receptor in synovial fluid and plasma in rheumatoid arthritis
- VernacularTitle:类风湿关节炎患者滑液和血浆中尿激酶型纤溶酶原激活物及其受体测定的临床意义
- Author:
Chunhua YANG
;
Feng HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Rheumatology
2001;5(3):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the levels of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in synovial fluid and plasma of patients with RA and investigate its clinical significance.Methods By using ELISA sandwich method,the levels of uPA and uPAR in plasma from 46 patients with RA and 8 with osteoarthritis (OA) were measured.Those in synovial fluid (SF) from 14 patients with RA,and plasma from 12 healthy subjects were measured as controls.Results The concentration of uPA and uPAR in SF from patients was significant higher than that in their plasma (P＜0.001,P＜0.01),and the concentration of uPA and uPAR in plasma of RA was also much higher than that in plasma of OA patients (P＜0.05,P＜0.000 1) and healthy subjects (P＜0.000 1,P＜0.001).There was no significance between plasma levels of RF＋ and RF－ in RA patients.The concentration of uPA and uPAR of plasma in RA correlated positively with CRP,RF and the number of swelling joints.Conclusion The levels of uPA and uPAR in SF and plasma are useful parameters for monitoring disease activity of RA.These findings suggest that the uPA and uPAR genes may play an important role via proteolytic damage of the extracellular matrix during the development of RA.
