Efficient Depletion of Multiple SARS-CoV mRNAs by a Single Small Interfering RNA Targeting The Leader Sequence
- VernacularTitle:针对leader序列的siRNA能够有效抑制SARS冠状病毒多个mRNA的表达
- Author:
Jian YE
;
Lixin LIU
;
Yuan XUE
;
Jing QU
;
Guangxia GAO
;
Rongxiang FANG
- Publication Type:Journal Article
- Keywords:
leader RNA;
RNAi;
SARS-CoV;
shRNA;
siRNA
- From:
Progress in Biochemistry and Biophysics
2007;34(10):1092-1100
- CountryChina
- Language:Chinese
-
Abstract:
Small interfering RNAs (siRNAs) can efficiently inhibit gene expression by sequence-specific RNA interference (RNAi). A common 5' leader sequence exists in the genomic RNA and all subgenomic RNAs of SARS-CoV, and is well conserved among various SARS-CoV strains, thus providing a preferable target for RNAi of SARS-CoV replication. Here efficient depletion of the SARS-CoV mRNAs by either a synthetic siRNA or DNA vector-derived short hairpin RNAs (shRNAs) targeting the leader sequence in mammalian cell lines were reported. The siRNA or shRNAs efficiently suppressed the expression of an EGFP reporter gene which contains the leader sequence at the 5' end. Both the siRNA and shRNAs efficiently knocked down the levels of leader-containing transcripts of three SARS-CoV genes encoding the spike protein, membrane protein and nucleocapsid protein were demonstrated. The results suggest that RNAi targeting the leader sequence is a potential efficient strategy for anti-SARS-CoV therapy.
