Effects of murine vascular endothelial growth factor (mVEGF) and anti- vascular endothelial growth factor (anti-VEGF) antibady on human umbilicus vein endothelial cell (HUVEC) proliferation
- VernacularTitle:鼠血管内皮生长因子及其抗体对内皮细胞增生影响的研究
- Author:
Jing LU
;
Lijuan ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Rheumatology
2001;5(3):160-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used 3 H-TaR incorporation.Results The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.
