Effect of T cell vaccination on cyclophosphamide-treated diabetes in NOD mice
- VernacularTitle:T细胞接种对环磷酰胺处理NOD小鼠糖尿病的影响
- Author:
Caifeng YAN
;
Zhili ZHANG
;
Qinghua GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Endocrinology and Metabolism
2001;17(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the prophylactic effect of T cell vaccination (TCV) on type 1 diabetes. Methods 6-week-old nondiabetic femele NOD mice were vaccinated intraperitoneally with the attenuated, activated T lymphocytes isolated from the spleen of 4-week-old nondiabetic NOD mice, 18-week-old newly diabetic NOD mice and 32-week-old long-term diabetic NOD mice. The incidence of cyclophosphamide-treated diabetes, inflammatory score of insulitis in NOD mice as well as T lymphocyte subset changes in spleen and thymus were determined after TCV. Results TCV was able to reduce the incidence of cyclophosphamide-treated diabetes, to alleviate insulitis, to increase the percentage of CD8+ T lymphocyte subset in spleen and CD4- CD8+ simple-positive T lymphocyte in thymus, and to decrease the percentage of IL-2R+ T lymphocyte and CD4+/CD8+ rate in spleen. Conclusion TCV may decrease host autoimmunity, which seems related to the changes of T lymphocyte subsets in spleen and thymus and prophylactic effect on diabetes.
