Eukaryotic Translation Factors Resided in PSI-induced Inclusions in PC12
- VernacularTitle:PC12细胞的PSI诱导性包含体富集了真核细胞翻译因子
- Author:
Xingan LI
;
Yingjiu ZHANG
;
Yihong HU
;
Ming CHANG
;
Tao LIU
;
Danping WANG
;
Lei ZHANG
;
Yu ZHANG
;
Linsen HU
- Publication Type:Journal Article
- Keywords:
proteasome inhibition;
PSI-induced inclusion;
eukaryotic translation factor
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(1):20-29
- CountryChina
- Language:Chinese
-
Abstract:
Lewy body (LB), an eosinophilic inclusion localized in the neuronal perikaryon, consists of a wide range of proteins, including the consistent organization and the selective composition. Treatment of PC12 cells with synthetic proteasome inhibitor (PSI) at 10 μmol/L for 48 hours induced the formation of inclusions, which were detected by eosin staining and immunostaining for α-synuclein. To investigate the potential new components of PSI-induced inclusions in vitro, pure intact inclusions were successfully obtained by fractionation and subjected to two-dimensional electrophoresis (2-DE) then analyzed with unequivocal matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Eukaryotic translation initiation factor 3 subunit 5 (eIF-3ε), eukaryotic elongation factor 2 (eEF-2) and mitochondrial elongation factor Tu (EF-Tumt) were identified. The results suggest that 3 eukaryotic translation factors recruited in PSI-induced inclusions may influence formation of the intermediate organelles following the inhibition of proteasomes.
