RP-HPLC study of resveratrol derivative (BTM-0512) in rat plasma and tissue distribution
- VernacularTitle:RP-HPLC法研究白藜芦醇衍生物(BTM-0512) 在大鼠血浆与组织中的分布
- Author:
Ning MA
;
Wenying LIU
;
Huande LI
;
Xinyu JIANG
;
Bikui ZHANG
;
Ronghua ZHU
;
Feng WANG
;
Yueliang XIE
;
Xiaoqian ZHOU
;
Xue WU
;
Daxiong XIANG
- Publication Type:Journal Article
- Keywords:
(E) -3,5,4'-trimethoxystilbene;
HPLC;
resveratrol
- From:
Acta Pharmaceutica Sinica
2007;42(11):1183-1188
- CountryChina
- Language:Chinese
-
Abstract:
This paper described a rapid and se nsitive HPLC method to analyze (E)-3, 5,4'-trimethoxystilbene (BTM-0512) in rat plasma and tissues. The analysis used a BDS Hypersil C18 analytical column (250 mm×4.6 mm ID, 5 μm) and acetonitrile / water as the mobile phase. The UV detection wavelength was 319 nm. Proteins were precipitated with acetonitrile and diethylstilbestrol as internal standard. The method was validated according to State Food and Drug Administration of China and ICH of Technical Requirements for Registration of Pharmaceuticals for Human Use Guidelines. The limit of interday precision values (%RSD) were in the range of 2.6% -5.1% and 2.4% -4.8%, respectively.Mean accuracy and absolute recoveries of BTM-0512 ranged from 95.3% - 100. 1% and 95.9% -100.9% for plasma and tissues, respectively. This method can be quite useful for BTM-0512 pharmacokinetic and tissue distribution studies, for purpose which multiple plasma and tissue samples can be analyzed quickly with high reproducibility.
