Research of Fusion Expression of Human β-Defensin-3 in Escherichia coli
- VernacularTitle:大肠杆菌中融合表达人β防御素-3基因的研究
- Author:
Yahua ZHAO
;
Laixiang XU
;
Jianjun ZHANG
;
Pengliang HUANG
;
Shaopeng XU
;
Mingjing SONG
- Publication Type:Journal Article
- Keywords:
hBD-3;
Fusion expression;
Repeated freezing and thawing;
Staphylococcus aureu s;
Antibacterial activity
- From:
Chinese Journal of Comparative Medicine
2007;17(11):670-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective To achieve the fusion expression of the entire human beta-defensin-3(hBD-3) gene. Method We synthesized two oligonucleotide primers accor ding to the codon preference of Escherichia coli. The gene was cloned into p GEX -4T-2 to establish the pGEX-4T-2-hBD-3 as the fusion expression vector by PCR. Transformed into E.coli strain DH5α, the express vector was induced an d ex pressed by IPTG. The fusion protein GST-hBD-3 was obtained by repeated cycles of freezing and thawing, cut by thrombin to attain the recombinant hBD-3 protei n. Result The result of the antibacterial peptide agarose diffu sion assay shows the antibacterial activity of the rhBD-3 against the S.aureu s exists, and it reaches 0.843U. Conclusion The fusion expr ession of the hBD-3 gene is successful.
