Isolation and activity of an α-amylase inhibitor from white kidney beans
- VernacularTitle:白豆中α-淀粉酶抑制剂的分离及其活性研究
- Author:
Xiaoqi ZHANG
;
Mingyan YANG
;
Yu MA
;
Jia TIAN
;
Jirong SONG
- Publication Type:Journal Article
- Keywords:
white kidney bean;
α-amylase inhibitor;
glycoprotein;
hypoglycemic activity
- From:
Acta Pharmaceutica Sinica
2007;42(12):1282-1287
- CountryChina
- Language:Chinese
-
Abstract:
An α-amylase inhibitor (α-AI) was isolated from white kidney beans (Phaseolus vulgaris. L) by ethanol fractional precipitation, ion exchange chromatography and gel filtration column chromatography. It was a homogeneity glycoprotein demonstrated by SDS-PAGE and gel filtration on CL-6B. The glycoprotein contained 88.2% protein and was rich in aspartic acid, glutamic acid, leucine, threonine and serine. The carbohydrate moiety was consisted of Man, Glc, Gal and Xyl in a mole ratio of 2.42∶1.50∶1.52∶1.00. The glycan and the core protein backbone was connected by O-linkage as determined by β-elimination reaction. The continuous oral administration of the α-AI (150 mg·kg-1·d-1 ) for 7 days can lower fasting blood glucose and 300 mg·kg-1 ·d-1 α-AI for 7 days can improve the sugar tolerance on alloxan-dependent diabetic model rats. The result showed the α-AI obtained from white kidney beans had good hypoglycemic effect on alloxan induced diabetic rats and may have high potential pharmaceutical value as a regulative digestive-starch degradation in patients suffering from diabetes.
