Expression of estrogen receptor β mRNA in endometrial carcinoma and its significance
- VernacularTitle:雌激素受体β mRNA在子宫内膜癌中的表达及其意义
- Author:
Hongwu WEN
- Publication Type:Journal Article
- From:
Chinese Journal of Obstetrics and Gynecology
2001;36(2):108-110
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the expression of estrogen receptor β (ERβ ) mRNA and its significance in endometrial carcinoma. Methods Thirty-six normal and 45 cancer tissue samples of endometrium were obtained, and the RNA was isolated from the tissues by the acid guanidium thiocyanate-phenol-chloroform extraction method. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify the ERβ exon 5 wild type mRNA (ERβ WT) and splicing variant mRNA (ERβ E5SV). Electrophoresis of polymerase chain reaction product was performed and the ratio of ERβ E5SV/ERβ WT was calculated. The sequencing of ERβ E5SV and ERβ WT was carried out by express DNA sequencer. Results ERβ E5SV and ERβ WT were both expressed in normal endometrium and endometrial cancer tissues. A region of 139 bp from base pair 812 to 950 (the hormone-binding domain) was found to be deleted in ERβ E5SV sequence as compared to ERβ WT. The quantity of ERβ E5SV was more than that of ERβ WT in every normal endometrium, and was less in every endometrial cancer, the ratio of ERβ E5SV/ERβ WT was 2.47±0.99 in normal endometrium, and 0.55±0.12 in endometrial cancer (P<0.000 1). The ratio of ERβ E5SV/ERβ WT decreased with the grade from 1 to 3 (P<0.01). Conclusion Overexpression of ERβ WT relative to ERβ E5SV may correlate with the genesis of endometrial carcinoma.
