Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons
- VernacularTitle:二脱氧肌苷引发培养的背根神经节神经元的形态学改变
- Author:
Huaxiang LIU
;
Zhen LIU
;
Xiangdong YANG
;
Lihong WANG
;
Zhenzhong LI
- Publication Type:Journal Article
- Keywords:
didanesine;
dorsal root ganglion;
neuron;
rat
- From:
Chinese Journal of Neuroanatomy
2008;24(1):8-12
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of didannsine(ddI)on the morphological alterations of dorsal root ganglion(DRG)neurons,dissoci-ated DRG cells from rat embryo were studied.DRG cells were cultured for 3 days and then treated with ddI for additional 3 claysin differ-ent concentrations(1μg/ml,5 μg/ml,10μg/ml and 20 μg/ml,respectively).Afarthat,DRG cells were processedformicrotubule as-soeiated protein 2(MAP2)labeling and observed under confocal laser scanning microscopy(CLSM).The results showed that both thenumber and length of neurites of the DRG cells after exposed to ddl significantly down-regulated in a dose-dependentmanner compared withcontrol group,thus suggesting that ddI may have inhibitory effects on neufite regeneration and outgrowth in dissociated DRG cultures.
