Sequence Variation in the Gp120 region of SHIV-CN97001 during in vivo Passage
- Author:
Qiang LIU
;
Guibo YANG
;
Yue MA
;
Chenli QIU
;
Jiejie DAI
;
Hui XING
;
Yiming SHAO
- Publication Type:Journal Article
- Keywords:
SHIV;
Passage;
Gp120;
Variation;
Sequence analysis
- From:
Virologica Sinica
2008;23(1):8-14
- CountryChina
- Language:Chinese
-
Abstract:
SHIV-CN97001 played an important role in assessing the immune effect and strategy of the AIDS vaccine which included genes of the predominant prevalent HIV-1 strain in China. In this study, SHIV-CN97001 was in vivo passaged serially to construct pathogenic SHIV-CN97001/rhesus macaques model. To identify variation in the gp120 region of SHIV-CN97001 during passage, the fragments of gp120 gene were amplified by RT-PCR from the plasma of SHIV-CN97001 infected animals at the peak viral load time point and the gene distances (divergence, diversity) were calculated using DISTANCE. The analysis revealed that the genetic distances of SHIV-CN97001 in the third passage animals were the highest during in vivo passage. It had a relationship between viral divergence from the founder strain and viral replication ability. The nucleic acid sequence of the V3 region was highly conservative. All of the SHIV-CN97001 strains had V3 loop central motif (GPGQ) and were predicted to be using CCR5 co-receptor on the basis of the critical amino acids within V3 loop. These results show that there was no significant increase in the genetic distance during serial passage, and SHIV-CN97001 gp120 gene evolved toward ancestral states upon transmission to a new host. This could partly explain why there was no pathogenic viral strain obtained during in vivo passage.
