Research on The Proteome Response of Acidithiobacillus ferrooxidans to Phosphate Starvation by SELDI-Protein Chip Technologies
- VernacularTitle:采用SELDI蛋白质芯片技术研究氧化亚铁硫杆菌对磷酸盐缺失的反应
- Author:
Zhiguo HE
;
Hui ZHONG
;
Qinghua LI
;
Guohua GU
;
Yuehua HU
;
Guiyuan LI
- Publication Type:Journal Article
- Keywords:
A cidithiobacillus ferrooxidans;
phosphate starvation;
proteomics;
SELDI Protein Chip
- From:
Progress in Biochemistry and Biophysics
2008;35(1):77-84
- CountryChina
- Language:Chinese
-
Abstract:
A cidithiobacillus ferrooxidans is a chemolithotrophic microorganism capable of using ferrous ions and sulphides as energy sources. This microorganism has an important role in the bioleaching of minerals. During this process, the bacteria are normally subjected to several stressing conditions, such as temperature changes, lack of nutrients or pH changes, which may affect the efficiency of the bacterial action. SELDI is a recent technology that allows for high-throughput proteomics studies. The Protein Chip SELDI technology was used to generate comparative protein profiles of A c idithiobacillus ferrooxidans grown under phosphate starvation or normal condition additionally adding Fe2+ as energy resource. There were 13 significantly differential expressed protein's peaks found by using SELDI Protein Chip technologies, which made a solid foundation for further isolation these low molecular proteins by adopting technologies such as HPLC etc.
