IMMUNOHISTOCHEMISTRY FOR Trk RECEPTORS IN RAT SCARPA'S GANGLION
- VernacularTitle:大鼠Scarpa神经节中Trk受体的免疫组织化学研究
- Author:
Yu CHEN
;
Yulin DONG
;
Tao LIU
;
Fuxing ZHANG
;
Jinlian LI
;
Yunqing LI
- Publication Type:Journal Article
- Keywords:
vestibular;
gangalion;
immunohistochemistry;
tyrosine;
kinase;
receptor;
rat
- From:
Chinese Journal of Neuroanatomy
2007;23(5):462-466
- CountryChina
- Language:Chinese
-
Abstract:
With the aim to examine the distribution of high-affinity neurotrophin receptors (tyrosine kinase receptors, TrkA, TrkB and TrkC)in the rat Scapa's ganglion ( vestibular ganglion, VG), Avidin-Biotin-peroxidase Complex ( ABC ) method of immunohistochemistry with diaminobenzidine (DAB) as the chromogen to identify the immunoreactivities was employed in the present study. The results showed that many VG neurons were immunoreactive to each Trk isoform. The receptors were localized in the neuronal somata. The intensity of immunoreactivity for each Trk receptor was different among neurons, ranging from weak, moderate to intense. For each individual Trk receptors, the labelled neurons were of different size; the result sfatistical of analysis showed that the mean areas for neurons immunoreative to TrkA, B and C were 330.8 ± 7.6, 303.89 ± 10.6 and 355.05 ± 8.3 μm2 , respectively. The present study provids morphological substrate for the important roles played by Trk receptors in maintaining the survival and stabilizing the phenotype of VG neurons.
