Protective effect of non-mitogenic human acidic fibroblast growth factor from renal ischemia-reperfusion injury*
- VernacularTitle:非促分裂型人酸性成纤维细胞生长因子对大鼠肾缺血再灌注损伤的保护作用
- Author:
Hua XU
;
Guangfa HAI
;
Hong XU
;
Jizhou XIANG
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor 2;
Reperfusion injury;
Kidney
- From:
Chinese Journal of Pathophysiology
2008;24(3):552-557
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of non-mitogenic human acidic fibroblast growth factor(nm-haFGF)on renal ischemia-reperfusion injury in rats.METHODS:Rat renal ischemia-reperfusion(I/R)injury was produced by removing the left kidney and subsequently clamping the right renal artery for 60 min followed by reperfusion for 24 h.5 min after reperfusion.different doses of nm-haFGF and haFGF(as positive control)were injected by lingual vein.24 h later,the samples of blood,urine and kidney were collected and the contents of malondialdehyde(MDA),blood urea nitrogen(BUN),creatinine(Cr)and superoxide dismutase(SOD)activity were detected.Histopathological changes were also observed.RESULTS:In the serum,SOD activity of all the nm-haFGF groups and the haFGF group increased significantly while the content of MDA decreased dramatically compared with the model group;The content of BUN and Cr aland haFGF group rose significantly compared with the model group,while MDA decreased dramatically.Histological examination showed that nm-haFGF markedly attenuates the renal edema,brush border's defluvium and cell necrosis induced by ischemia-reperfusion.CONCLUSION:nm-haFDF could resist the renal injury induced by ischemia-reperfusion in rats.
