NOK Activates STAT3 Signaling by a JAK2-Dependent Mechanism
- VernacularTitle:NOK通过JAK2依赖性方式激活STAT3信号通路
- Author:
Yinghua LI
;
Yu RONG
;
Zhijie CHANG
;
Li LIU
- Publication Type:Journal Article
- Keywords:
NOK;
JAK2;
STAT3;
synergistic effect;
co-immunoprecipitation;
signaling activation
- From:
Progress in Biochemistry and Biophysics
2008;35(2):143-150
- CountryChina
- Language:Chinese
-
Abstract:
Novel oneogene with kinase-domain (NOK) can activate multiple mitogenic signaling pathways including the janus kinases(JAK) and signal transducer and activator of transcription proteins (STAT). It was showed that NOK specifically and physicallyinteracted with STAT3 in human embryo kidney 293T (HEK293T) cells. In addition, NOK could directly interact with most of the STAT3 subdomains except coiled-coil and C-terminal domains. Removing ectodomain and transmembrane domain of NOK markedly enhanced its intermolecular interaction with STAT3. Also, NOK could co-immtmoprecipitate with JAK2. in vivo. Importantly, co-expression of NOK and JAK2 produced a synergistic effect on NOK-mediated STAT3 activation, while inactivating the kinase domain of JAK2 completely prevented this synergistic effect. Overall, the results indicated that NOK might complex with both STAT3 and JAK2 and activate STAT3 signaling by a JAK2-dependent mechanism.
