Determination of betamethasone in human plasma by liquid chromatography with tandem mass
- VernacularTitle:LC-MS/MS法测定人血浆中倍他米松
- Author:
Tingting QU
;
Rui ZHANG
;
Benjie WANG
;
Xiaoyan LIU
;
Guiyan YUAN
;
Ruichen GUO
- Publication Type:Journal Article
- Keywords:
betamethasone;
LC-MS/MS;
pharmacokinetics;
compound betamethasone injection
- From:
Acta Pharmaceutica Sinica
2008;43(4):402-407
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm ×3.9 mm ID, 5 μm) with methanol-water with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3→355.2 for betamethasone and m/z 361.3→343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The validation study demonstrated excellent precision and accuracy across the calibration range of 0.5 - 80.0 injection in healthy Chinese volunteers.
