Effect of berberine on cyclin dependent kinase 9 and cyclin T1 expressions in type 2 diabetic rat kidney
10.3867/j.issn.1000-3002.2008.02.001
- VernacularTitle:小檗碱对2型糖尿病大鼠肾组织细胞周期蛋白依赖激酶9和细胞周期蛋白T1表达的影响
- Author:
Jiyin ZHOU
;
Shiwen ZHOU
- Publication Type:Journal Article
- Keywords:
berberine;
cyclin dependent kinase 9;
cyclin T1;
diabetic nephropathy
- From:
Chinese Journal of Pharmacology and Toxicology
2008;22(2):81-87
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate cyclin dependent kinase 9(Cdk9) and cyclin T1 protein expressions in diabetic rat kidney and the effect of berberine on them. METHODS Type 2 diabetes mellitus (T2DM) rats were induced by injection (ip) with diet for 16 weeks. From week 17 to 32, diabetic rats were given berberine 75, respectively. The kidney tissue structure was observed with hematoxylin/eosin (HE) staining, kidney to body weight ratio was calculated, and Cdk9 and cyclin T1 expressions were examined by immunohistochemistry. RESULTS Compared with control rats, the volume of diabetic model rat glomerulus accreted, some intercapillary cells proliferated and mesangial region expanded, both glomerular basement membrane and renal tubular basement membrane thickened. Treatment with diabetic nephropathy symptom. The diabetic kidney to body weight ratio protein expressions in diabetic kidney to near control level. CONCLUSION Berberine regulates Cdk9 and cyclin T1 protein expressions in diabetic kidney which may partly contribute to ameliorate nephropathy complication induced by STZ and the high-carbohydrate/high-fat diet.
