Protective effect of astragalus polysaccharide on mitochondria and lysosome in H2O2 -stressed skin fibroblasts
- VernacularTitle:黄芪多糖对过氧化氢刺激皮肤成纤维细胞中线粒体和溶酶体的保护作用
- Author:
Xiaoli YUE
- Publication Type:Journal Article
- Keywords:
Oxidative stress;
Mitochondria;
Lysosomes;
Astragalns polysaccharide
- From:
Chinese Journal of Pathophysiology
2008;24(4):777-782
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To verify the protection of astragalus polysaecharide (APS) on H2O2 - stressed skin fibro-blasts. METHODS: A model of acute H2O2 stress in primary skin fibroblast was used at concentration of 0.5 mmol/L by 30 rain incubation. Dose responses of APS on cell survival was measured by MTI, cell death was evaluated by DAPI, and effect of APS on mitochondria, mitochondrial membrane potential and lysesome stabilization were measured by confocal microscopy. RESULTS: APS improved cell survival in a dose -dependent manner, starting at 0. 5 mg/L and with a maximum at 1 mg/L. Moreover, APS inhibited mitochondrial membrane potential collapse, protected mitechondrial morphology and stablized lysosomal membrane. CONCLUSION: The results suggest the existence, at the mitochondria-lysosome level, of a new pathway of apoptotic regulation by APS. This might constitute a new therapeutic target where oxidative stress and lysesomal impairment are involved.
