The effect of TNF-related apoptosis-inducing ligand on the activity of nuclear kappa B in prostate cancer cell line PC-3M
- VernacularTitle:肿瘤坏死因子相关凋亡诱导配体在前列腺癌细胞株PC-3M中对核因子kappa B活化的影响
- Author:
Wei ZHONG
;
Wei HE
;
Wang MA
;
Keliang ZHANG
;
Shaozhong WEI
;
Zhaohui CHEN
;
Xiaochun CHEN
;
Fuqing ZENG
;
Chuanguo XIAO
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Tumor necrosis factor;
NF-kappa B;
Tumor necrosis factor-related apoptosis-inducing ligand
- From:
Chinese Journal of Pathophysiology
2008;24(5):966-971
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the activation and inactivation of nuclear factor kappa B(NF-κB)when tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)is applied to induce the apoptosis of androgen-independent prostate cancer cell line PC-3M.METHODS:After the treatment of TRAIL or LPS at different doses,we tested the nuclear translocation of NF-κB by cell immunohistochemical staining and electrophoretie mobility shift assay(EMSA),and evaluated the level of IκB by RT-PCR under pyrrolidine dithiocarbamate(PDTC)treatment.RESULTS:EMSA and cell immunohistochemical analysis showed that the translocation of NF-κB was significantly activated when PC-3M cells were treated with TRAIL or LPS(P＜0.05).The pretreatment of PDTC upregulated the expression of IκB and blocked the nuclear translocation of NF-κB.CONCLUSION:TRAIL remarkably stimulates the activation of nuclear NF-κB in androgen-independent prostate cancer cells.On the other hand,the translocation of NF-κB can be significantly and efficiently inhibited in PC-3M cells by pretreatment with PDTC.The increased expression of IκB might be a clue for this inhibition,which means the possible way to enhance the effect of TRAIL in the apoptosis of prostate cancer cells.
