Enzymatic Synthesis of Endomorphin by Immobilized Papain on Sodium Alginate-Chitosan
- VernacularTitle:海藻酸钠-壳聚糖固定化木瓜蛋白酶催化内吗啡肽的合成
- Author:
Ping HE
;
Zhuolie HUANG
;
Chunyi LI
;
Weijia CHEN
;
Guanghong WU
;
Zhizhan CHU
- Publication Type:Journal Article
- Keywords:
endomorphin;
immobilized papain;
organic solvent
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(5):426-431
- CountryChina
- Language:Chinese
-
Abstract:
The enzymatic synthesis of endomorphins were carried out by immobilized papain on sodium alginate-chitosan (IPSAC). The reaction has been carried out in two steps. First, Trp-Phe-NH2 was obtained by Boc-Trp-OH coupling with Phe-NH2 using IPSAC catalyst in microaqueous acetonitrile system with a 27.8 % yielding as determined by high performance liquid chromatography (HPLC). The catalytic properties of IPSAC were examined by studying the dependence of pH, ionic strength, solution content, reaction temperature,enzyme loading and reaction time over the yields. The results of orthogonal experiments indicated that pH was the most significant factor that influenced this synthesis. Second, Boc-Tyr-Pro-OMe and Trp-Phe-NH2 were suspended into the microaqueous acetonitrile to produce Tyr-Pro-Trp-Phe-NH2 (endomorphin-1) with a 35.2% yield.
