Expression profiles of iron metabolism-related genes in macrophages infected by Salmonella typhimurium
- VernacularTitle:鼠伤寒沙门菌感染的巨噬细胞铁代谢相关基因表达谱
- Author:
Xin PAN
- Publication Type:Journal Article
- Keywords:
macrophages;
Salmonella typhimurium;
iron metabolism
- From:
Medical Journal of Chinese People's Liberation Army
2008;33(5):502-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the iron metabolism in host-pathogen interplay.MethodsA total of 13 expression profiles of iron metabolism related genes of RAW264.7 murine macrophages uninfected or infected with Salmonella typhimurium were stuldied by real-time PCR.Results The live wild-type Salmonella typhimurium induced the expression of the transferrin receptor(Tfr1)in host cell macrophages,which resulted in a sustained increase of the labile iron pool inside the host oell 1h or 24h after infection.Gene expression analysis showed that wild-type Salmonella typhimurium drove an active iron acquisition program with induction of ferrireductase(Steap3),iron membrane transporter Dmtl,and iron regulatory proteins(Irpl and Irp2),and a little of iron efflux change through ferriportin(Fpnl).On the other hand,the Salmonella mutant strain spiC-used in present studies did not cause an increase mRNA level for Tfrl at 1h or 24h,but led to an increase mRNA level for Epnl at 24h as compared with 1h. The assessment was that the labile iron pool decreased after infection with spiC-Salmonella for 24h.Conclusion Wild-type Salmonella typhimurium appears to drive an active transferrin-mediated iron uptake program after infection 1h or 24h.The spiC-Salmonella mutant strain used in our studies shows an increased iron efflux from infected cells at 24h as comparde with 1h.
