The research of peripheral blood regulatory T cell in patients with idiopathic thrombocytopenic purpura
- VernacularTitle:特发性血小板减少性紫癜患者外周血调节性T细胞的研究
- Author:
Yi ZHU
;
Changlin WU
;
Jiancheng XUE
- Publication Type:Journal Article
- Keywords:
Purpura,Thrombocytopenic,Idiopathic;
T Lymphocytes,Regulatory;
Research
- From:
International Journal of Laboratory Medicine
2008;29(6):490-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of peripheral blood CD4+CD25+ regulatory T cells (Tr) in patients with idiopathic thrombocytopenic purpura (ITP), and to analyze its role in the pathogenesis of ITP.Methods Anticoagulated venous blood was collected from ITP patients (ITP group,n=35) and healthy controls (healthy control group,n=35). T lymphocytes were isolated and purified with human CD3+ T cell enrichment columm. The percentage of peripheral blood CD4+CD25+ regulatory T cells was detected by immunofluorescence staining (PE-anti-CD4 monoclone antibody and FITC-CD25 monoclone antibody) and bicolor flow cytometry by CD25/CD4 gating.Results The number and constituent ratio of CD4+CD25+T cell were significantly lower in ITP group than those of healthy control group (P<0.05).Conclusion There is peripheral blood celluar immunological function disorder in ITP patients, and decrease of CD4+CD25+ T cell population may be involved in the pathogenesis of ITP.
