Appraisal of determination of serum type Ⅳ collagen protein with latex-enhanced immunoturbidimetry
- VernacularTitle:胶乳增强免疫比浊法测定血清Ⅳ型胶原蛋白的使用评价
- Author:
Haimin WANG
;
Jianhua WU
- Publication Type:Journal Article
- Keywords:
Nephelometry and Turbidimetry;
Collagen,Type Ⅳ;
Liver Cirrhosis;
Evaluation Studies
- From:
International Journal of Laboratory Medicine
2008;29(6):508-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To appraise determination of serum type Ⅳ collagen protein with latex-enhanced immunoturbidimetry.Methods The evaluation test was performed including precision, linearity determination, prozone reaction, correlation, recovery test and interference test.Results The intra-batch CV% was within 2.47%-3.48%, inter-batch CV% was within 3.46%-4.07%, and recovery rate was within 96.2%-99.3%. There was a good linearity, and the tolerance limit was 800 ng/mL.Regression equation:Y=1.011 8X-1.788 3;detection correlation of different concentrations of standard preparations:r2=0.999 9,Y=1.003 1X-1.236 2.The assay had a very good anti-interference performance to bilirubin, cruorin, and triglyceride without exception.Conclusion Determination of serum type Ⅳ collagen protein attains the demand of the clinic with latex-enhanced immunoturbidimetry in fully automatic biochemical analyzer.
