Effect of berberine on positive transcription elongation factor b expression in diabetic rat retinas
- VernacularTitle:小檗碱对2型糖尿病大鼠视网膜正性转录延伸因子b表达的影响(英文)
- Author:
Jiyin ZHOU
;
Shiwen ZHOU
- Publication Type:Journal Article
- Keywords:
berberine;
diabetic retinopathy;
positive transcription elongation factor B;
cyclin-dependent kinases 9;
cvclin T1
- From:
Chinese Journal of New Drugs and Clinical Remedies
2008;27(4):243-248
- CountryChina
- Language:Chinese
-
Abstract:
AIM To inVestigate the positiVe transcription elongation factor b(P-TEFb)expressjon and the effect of berberine on diabetic retina of the rat.METHODS Type 2 diabetes mellitus rat models were diabetic control rats(group A)that neither received STZ nor the high-carbohydrate/high-fat diet;16-wk diabetic rats without any drug treatment(group B);diabetic rats treated with berberine at a dose of 75,150 or staining and P-TEFb(cyclin-dependent kinases 9(CDK9)and cyclin T1)protein expression was detected by immunohistochemjstry.RESULTS The retinas of control group were thicker than those of other 6 groups.After thickness but no difference in retinal structure among all groups.Middle-.high-dose berberine and rosiglitazone fenofibrate showed no effect on CDK9 and cyclin T1 expression.CONCLUSION Berberine modulating P-TEFb level in diabetic retina may probably be one of the mechanisms to ameliorate retinopatby induced by STZ and the high-carbohydrate/high-fat diet.
