Optimal preparation conditions of porous chitosan microspheres
- VernacularTitle:多孔壳聚糖微球的最佳制备条件
- Author:
Zhizhou LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;12(14):2770-2772
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Most of the natural chitosan are shaped as powder and have small specific surface area.It is difficult to reclaim both vector and adsorbate by chitosan,thus limiting its application as an adsorbing vector.OBJECTIVE:To analyze the optimal preparation technology of porous chitosan microspheres.DESIGN,TIME AND SETTING:Repeated-measures experiment was carried out in the laboratory of Applied Chemistry,Shaanxi University of Technology from November 2006 to May 2007.MATERIALS:Chitosan with a deacetylation degree of 95.62%.METHODS:The reverse liquid phase suspension polymerization method was adopted in this study using cyclohexane as porogenic agent and glutaraldehyde as crosslinking agent.Cyclohexane and cutaraldehyde were added continuously,with the alterations of reaction temperature,stirring speed and extraction time.MAIN OUTCOME MEASURES:The influence on the preparation of porous chitosan microspheres by the dosage of cyclohexane and glutaraldehyde,reaction temperature,stirring speed and extraction time was observed.RESULTS:The optimal conditions of preparing porous chitosan microspheres,in which 90% particle diameters were 3-12 μm,included:reaction temperature 60-70℃.medium stirring speed 100-120 r/min,extraction time no less than 48 hours.CONCLUSION:The preparation method used in this study has short time-consuming,the prepared chitosan microspheres are characteristic as micron-grade diameter,narrow distributing particle size and porosity.
