Activities of myocardial mitochondria enzyme complexes of respiratory chain in rats after living high-training low Time for the acclimatization of mitochondria
- VernacularTitle:高住低训模型大鼠心肌线粒体呼吸链酶复合物的活性:线粒体习服过程所需时间分析
- Author:
Yongchun LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;12(24):4780-4783
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: Previous studies have suggested living high-training low (HiLt) is effective in 4-6 weeks. We know little about the acclimatization time of mitochondria and activities of enzyme complexes during the process of HiLo. OBJECTIVE: Using rat models under simulated HiLt condition, to observe the dynamic changes in mitochondrial respiration and activities of enzyme complexes during the process of HiLt under hypoxia, so as to investigate the acclimatization time of mitochondria and enzyme complexes of respiratory chain needed during HiLo. DESIGN: Grouping and controlled animal trial. SETTING: Exercise Physiology Laboratory of Physical Education Institute of Liaoning Normal University. MATERIALS: Forty healthy adult SD rats, either male or female, were provided by the Animal Experimental Center.of Dalian Medical University. The animal procedure was accorded with the animal ethics. The hypoxia partial pressure machine (HTS) was from Hypoxico Company of America, and Oxygen partial pressure monitor was TOXIBLAEPGM-36 from America. METHODS: After 1 week of adaptive feeding, the rats were randomly divided into normoxic control group, HiLo 1, 2, 3, and 4 weeks groups with 8 rats in each group. HiLt groups lived at 2 500 m (O2 content was around 15.4%) and swam at 1 300 m (O2 content was around 19.4%), 1 hour once for 4 weeks. MAIN OUTCOME MEASURES: The activities of enzyme complexes Ⅰ-Ⅳ in the respiratory chain of mitochondria were measured using UV-spectrophotometer, and the method of Bradford was used to quantify protein of mitochondria. RESULTS: The activities of enzyme complexes Ⅰ-Ⅳ of respiratory chain reduced significantly compared with control group in the first week (P < 0.05-0.01); the activities of enzyme complexes Ⅲ recovered completely in the second week, which was not significantly different from the control group (P > 0.05). The activities of enzyme complexes Ⅰ-Ⅳ recovered completely in third week compared with the control group (P > 0.05). There was no significant difference in protein quantity of mitochondria between HiLo 1 and 2 weeks groups and control group (P > 0.05), but significant differences were found between HiLo 3 and 4 weeks groups and control group (P < 0.05) CONCLUSION: The acclimatization of the activities of enzyme complexes Ⅰ-Ⅳ in the respiratory chain will complete in three weeks in rats during the process of HiLo.
