Comparative Proteome Analysis of Plasma Membrane from Different Differential Nasopharyngeal Carcinoma Cell Lines
- VernacularTitle:不同分化程度的鼻咽癌细胞系质膜差异蛋白质组分析
- Author:
Tingting SHENG
;
Lijun ZHANG
;
Xiaohui LIU
;
Xiaofang JIA
;
Jixian XIONG
;
Quanyuan HE
;
Rui CAO
;
Xia PENG
;
Ni SHI
;
Songping LIANG
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma;
differentiation;
plasma membrane;
nlass spectrometry;
proteome
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(8):712-718
- CountryChina
- Language:Chinese
-
Abstract:
A subcellular proteomic method was applied to investigate the protein expression profiles of nasopharyngeal carcinoma (NPC) cell lines,CNE1 and CNE2,at various differentiation levels.Plasma membrane (PM) proteins were obtained by Percoll density grade centrifugation and subjected to twodimensional electrophoresis (2DE) followed by PDQuest software analysis.Nine proteins expressed with more than two folds difference were identified by MALDI-TOF-TOF,of which functions involved in cell differentiation,signal transduction,and metabolism.Half of these proteins,such as galectin-1 and annexin Ⅱ,were analyzed with real-time quantitative PCR or Westem blotting.We have tested a proteomic method to study differentiated NPCs at different levels and found several proteins that might be related to their biological characteristics.
