Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env
- Author:
Jianhui NIE
;
Chuntao ZHANG
;
Huihui CHONG
;
Xueling WU
;
Chuyu LIU
;
Yu WU
;
Chenyan ZHAO
;
Linqi ZHANG
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
Env;
Modification;
Neutralization assay;
ELISPOT
- From:
Virologica Sinica
2008;23(4):233-246
- CountryChina
- Language:Chinese
-
Abstract:
Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around Nab epitopes and deletions of variable regions in env.The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γELISPOT.Overall,five mutants(dWt,M2,M5-2,M5-1 and dM7)induced highed neutralization activities for both pseudoviruses than plasmid Wt,while only two of the mutants(dWt and M5-2)showed significant differences(P<0.05).Two mutants(M2 and dM2)induced more Env-specific T cells than plasmid Wt.Statistically however,significance was only reached for mutant M2.Thus,properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
