Characterization and Amino Acid Sequence of y3,an Antiviral Protein from Mushroom Coprinus comatus
- VernacularTitle:毛头鬼伞(Coprinus comatus)中一种抗病毒蛋白y3特性和氨基酸序列分析
- Author:
Liping WU
;
Zujian WU
;
Dong LIN
;
Fang FANG
;
Qiying LIN
;
Lianhui XIE
- Publication Type:Journal Article
- Keywords:
mushroom;
Coprinus comatlzs;
protein;
antiviral activity;
clone
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(7):597-603
- CountryChina
- Language:Chinese
-
Abstract:
Many proteins from mushrooms can be used as potential antiviral agents and in plant protection.An antiviral protein,designated as y3,Was isolated from fruiting bodies of the fungus Coprinus comatus by fastflow ion-exchange column chromatography coupled with high-resolution molecular sieve chromatography.This glycoprotein was detected in both fruiting body and mycelium by Western blot analysis.According to its Nterminal sequence.an amino acid sequence and a partial cDNA sequence of the y3 gene were obtained.Protein y3 at 2.0μg/ml achieved 50.0% inhibition of tobacco mosaic virus(TMV,20μg/ml)lesions in Nicotiana glutinosa leaves.It also inhibited multiplication of TMV in Nicotiana tabacum Var.K326.
