Amplification and Characterization of Bull Semen Infected Naturally with Foot-and-mouth Disease Virus Type Asial by RT-PCR
- Author:
Junjun SHAO
;
Huiyun CHANG
;
Tong LIN
;
Guozheng CONG
;
Junzheng DU
;
Jianhong GUO
;
Huifang BAO
;
Youjun SHANG
;
Yamin YANG
;
Xiangtao LIU
;
Zaixin LIU
;
Jixing LIU
- Publication Type:Journal Article
- Keywords:
Bull;
Foot-and-mouth disease (FMD);
Foot-and-mouth disease virus (FMDV);
RT-PCR;
Semen
- From:
Virologica Sinica
2008;23(5):378-382
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus (FMDV), but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease (FMD). This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group.
