Association of different susceptibilities to morphine with the expression of 5-HTT and 5-HT1AR mRNA in brain regions of SD rats
- VernacularTitle:SD大鼠吗啡依赖易感性差异与脑内5-羟色胺转运体和5-羟色胺1 A受体mRNA表达的关系
- Author:
Hongxian CHEN
;
Ying WANG
;
Minjie YE
;
Ruiling ZHANG
;
Changqi LI
;
Wei HAO
- Publication Type:Journal Article
- Keywords:
susceptibility;
morphine dependence;
5 -hydroxytryptamine transporter;
5-hydroxytryptamine 1 A receptor;
conditioned place preference
- From:
Journal of Central South University(Medical Sciences)
2008;33(9):775-783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism for the different CPI susceptibili-ties. Methods Using a conditioned place preference (CPP) model, rats were selected into high and low preference groups. Using in situ hybridization, we examined the mRNA expression of 5-hydroxytryptamine transporter (5-HTT) and 5 -hydroxytryptamine 1 A receptor (5-HT1 AR) in 3 cruci-al regions in addiction, namely the ventral tegmental area (VTA) , the nucleus accumbens (NAc) ,and the medial prefrontal cortex (mPFC) , during the dependence and withdrawal. Results During dependence state, the expression of 5-HTT mRNA in each of the regions in the high preference group was significantly lower than that of the low preference group, while higher expression of 5-HT1AR mRNA in each of the regions in the high preference group than that of the low preference group was found (P<0.05). During withdrawal state, the expression of 5-HTT mRNA in each of the regions in high preference group was significantly higher than that of the low preference group, while lower expres-sion of 5-HTIAR mRNA in each of the regions in the high preference group than that of the low prefe-rence group was found (P<0.05). Conclusion 5-HTT and 5-HT1 AR may play a role in diffe-rences in susceptibility to morphine.
