Effect of β-asarone on expression of ECV340 cell adhesion molecules induced by β-amyloid peptide
- VernacularTitle:β-细辛醚对Aβ痴呆损伤过程中ECV304细胞黏附分子表达的影响
- Author:
Yong JIANG
;
Yuping HE
;
Yongqi FANG
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
β-Amyloid;
ECV304 cells;
CD106;
CD62P;
CE62E
- From:
Chinese Traditional Patent Medicine
2008;30(10):1423-1427
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of β-asarone on vascular endotheliam and adhesion molecule expression of endothelium induced by β-amyloid peptide from Alzheimer's disease and to estimate the injury repair.METHODS:Cultured ECV304 cells were incubated with freshly solublizeal Aβ1-42 and the mixture of Aβ1-42 and β-asarone,the expression of three central adhesion molecules,CD106,CD62P,CE62E and Ca2+ concentration were examined and apoptosis was recorded by Flow eytometry.Test viability of cells by MTT methods.RESULTS:The results showed that in model group and treated group,ligation of endothelial CD106,CD62P,CE62E,markers for endothelial cell activation and Ca2+ concentration,leads to a lot of release.The livability decreased and the apoptosis increased.Further more,simultaneous treatment of ECV304 cells with β-asarone resulted in the decrease significandy in these three adhesion molecules described above and Ca2+ concentration as well as the livability upper and apoptosis lower.CONCLUSION:CD106,CD62P,CE62E,important inflammational factor of Aβ-induced endothelial injury,may be promotion of the inflammatory scade in vascular endothelial.β-asarone may protect ECV304 cell apoptosis by regulate Ca2+ and expression of cell surface markers.
