Detection of class Ⅰ integron in multi-resistant Enterobacter cloacae
- VernacularTitle:多重耐药阴沟肠杆菌Ⅰ类整合子的检测
- Author:
Fuhua LUO
;
Xiang LI
;
Yong WU
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
Genes,MDR;
Integrons
- From:
International Journal of Laboratory Medicine
2008;29(9):784-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and effect of class Ⅰ integron in 40 strains of clinical multi-resistant Enterobacter cloacae. Methods Kirby-Bauer method was used to analyze the drug resistance of 40 strains of multi-resistant Enterobacter cloacae and the drug resistance gene was analyzed by polymerase chain reaction (PCR). Results 28 strains (70.0%) of 40 clinical isolates were observed to contain class Ⅰ integron. Drug resistance test showed their resistance rate to 10 kind of an-tibaeterials as follow: 7.5% to imipenem, 5.0% to meropenem, 75.0% to piperacillin-tazobactam,55.0% to amikacin, 12.5% to cefepime, 52.5% to ceftazidime, 55.0% to cefotaxime, 90.0% to ceftriaxone;81.8% to ticarcillin-clavutanic acid, and 85.0% to ciprofloxacin. Conclusion This study shows that the resistance situation of Enterobacter cloacae isolates is very serious, and class Ⅰ integron may play an important role in the muhidrug resistant mechanism.
