Effects of red clover extract on the activation and proliferation of mouse T lymphocytes and the NO secretion of mouse macrophages
- VernacularTitle:红车轴草提取物对小鼠淋巴细胞活化与增殖及巨噬细胞分泌NO的影响
- Author:
Zhi YANG
;
Xiuyan HUANG
;
Yaoying ZENG
- Publication Type:Journal Article
- Keywords:
red clover extract;
lymphocyte;
macrophage;
proliferation;
activation;
nitric oxide
- From:
Acta Pharmaceutica Sinica
2008;43(10):1019-1024
- CountryChina
- Language:Chinese
-
Abstract:
The study investigated the effects of red clover extract (RCE) on mouse T macrophages and lymphocytes in vitro. The cell toxic effect of RCE was estimated by MTT assay. Multiple-fluorescence staining plus flow cytometry were used to detect the effect of RCE on CD69/CD25/CD71 expression of mouse T lymphocytes stimulated by Con A; CFDA-SE staining plus flow cytometry were used to analyze the effect of RCE on proliferation of T lymphocytes activated by Con A; The effect of RCE on nitric oxide(NO) secretion of mouse macrophages stimulated by lipopelysaccharide (LPS) for 24 h was assayed by Griess reagent system. We found that RCE had potent anti-inflammatory effects on mice. RCE had littl ecell toxic effect on mouse lymphocytes and macrophages. RCE strongly inhibited the excessive production of inflammatory mediators ( NO, CD69, CD25, CD71 ), in a dose-dependent manner, like cyclosporine A injection. RCE could inhibit proliferation of CD3<'+> T lymphocytes. These data suggested that RCE migh texhibit anti-inflammatory effect by inhibiting the activation and proliferation of mouse lymphocytes and the NO secretion of mouse macrophages.
